FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14845577
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC2211861.4899117764166392Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATA187300.12616552391328406Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT707850.077.4614649
AAGAGCG743250.074.2497948
GATCGGA746400.073.761142
TCGGAAG745900.073.441494
AGATCGG759150.072.323271
GAAGAGC787050.070.283457
ATCGGAA828300.066.296063
CGGAAGA936850.059.106585
GGAAGAG973450.057.1745766
GGGGACC61350.038.0722431
GGGACGA126850.030.0789341
GGGATGA241100.029.935951
GGGGACT83600.029.5012071
GGGCCGA78950.029.033681
GGGGTTT108600.028.4542351
GGGAAAA582700.027.4368171
GGGGTAA101150.027.3945791
GGGGACG115900.027.225321
GGGGGAC89800.026.65661
GGGCGAA197300.025.9931831
Produced by FastQC (version 0.11.5)