FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10519966
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC608430.5783573825238599Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA237400.067.448952
GGGGGAC78800.063.884741
GGGGGCC64050.063.873961
AGATCGG250150.063.6789171
AGAGCGT248700.063.3698439
AAGAGCG264250.059.969858
TCGGAAG265550.059.6484154
GAAGAGC279550.056.7910657
GGGGACC64600.054.010281
GGGGACG88300.052.9040641
ATCGGAA305300.052.099523
CGGAAGA321550.050.2064675
GGGGGTC42400.049.6130641
GGGCCGG69400.049.3340071
GGGGACT60350.049.039581
GGGGCCG64850.047.5382081
GGGGATC63250.047.479231
GGAAGAG364100.044.2993626
GGGGCTC34300.043.5649221
GGGGACA100950.043.2567631
Produced by FastQC (version 0.11.5)