FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14153935
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC1375090.971524879830238Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATA142340.10056567307960648Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT451450.081.6419759
AAGAGCG453700.081.555918
TCGGAAG455500.081.1217044
GATCGGA461300.080.7313842
GGGGGCC84850.075.824421
ATCGGAA491750.075.353583
GAAGAGC501050.074.0801247
AGATCGG521850.071.17811
CGGAAGA550900.067.810025
GGGGGAC102500.065.881451
GGGGACC91150.054.7481041
GGAAGAG689100.054.5891346
GGGGACG122150.050.5921861
GGGGCGA117800.045.5641981
GGGGAGC142250.044.922191
GGGGCCC57100.043.1593172
GGGCCGA73950.042.3723831
GGGGACT80750.042.1276861
GGGGCGC82000.042.0162281
GGGGCCG111550.040.444371
Produced by FastQC (version 0.11.5)