FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19879697
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA2265041.1393735025237055TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG731150.062.4630131
GATCGGA728850.062.461332
AGAGCAC728350.060.990669
TCGGAAG792200.056.550974
GAAGAGC828700.053.815627
GGAAGAG834300.053.6116376
CGGAAGA852000.052.531655
ATCGGAA884600.051.0781333
AAGAGCA911800.049.0143248
CCGATCT205700.041.486686
TCCGATC205800.041.325395
GATCTAG28100.036.1228268
CGATCTG201500.035.154387
GATCTGG185000.031.3921288
CTTCCGA279850.031.0892643
ATCTGGG143450.030.5275549
CGATCTA49000.027.6697677
CTACACG75350.026.2686141
TTCCGAT375500.023.0349464
TACACGA88900.022.5910762
Produced by FastQC (version 0.11.5)