FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14241569
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC567480.39846733179469196Illumina Single End PCR Primer 1 (96% over 33bp)
GGGTAAGTAAAACGGTTTTTTGAGTAACAAAAGTTTCCCAAAAACTATAT246490.1730778399486742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC183900.058.327181
GGGGACG180100.054.1215361
GGGGGCC178250.051.8758431
AGAGCGT285100.047.296229
GGGGACC154400.046.5020871
GGGGGAT168800.045.757441
GGGGAGC225600.045.2634961
GGGGGGA183400.044.527021
GGGGAGA250050.044.463111
GGGGACT101950.044.318531
GGGGGCT104550.043.840711
GGGGCGA196700.043.6179161
GGGGATC139000.043.1494451
GATCGGA321400.042.9588782
GGGGGTC82100.042.5783121
GGGGGGC186450.042.2427331
AGATCGG332300.041.402021
GGGGAGG197250.040.5181051
GGGGGCA154200.039.883751
GGGGGAG197200.039.351511
Produced by FastQC (version 0.11.5)