FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17093336
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA3321371.9430788700344976TruSeq Adapter, Index 27 (97% over 39bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC178150.063.817815
GATCGGA1069200.063.44562
AGATCGG1092950.062.21341
AGAGCAC1078750.061.5467539
TCGGAAG1132900.059.2762684
ATCGGAA1174000.057.496533
GGAAGAG1171400.057.1388556
CGGAAGA1197350.056.057125
CCGATCT203450.055.846936
GAAGAGC1203350.055.52967
AAGAGCA1219150.054.7382558
CGATCTG203050.046.2781567
CTTCCGA271550.042.9601973
CGATCTA48700.041.0917057
GATCTGG192050.039.188138
TTCCGAT295750.038.979844
GATCTAG43250.036.546178
ATCTGGG166300.034.6615229
CTCTTCC400600.030.8955971
TCTTCCG399450.030.7301562
Produced by FastQC (version 0.11.5)