FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17093336
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATC2423841.4180028989075042Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATA178380.10435645797871171Illumina Single End PCR Primer 1 (96% over 33bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCC197850.072.326161
GGGGGAC238850.069.931531
GGGGACG210400.061.324581
AGAGCGT1038950.059.3059549
GATCGGA1081500.057.496142
GGGGGCA158050.056.7648541
AGATCGG1100350.056.3954771
GGGGGCT130150.056.2278331
AAGAGCG1109150.055.8461768
TCGGAAG1144250.054.1454354
GGGGGTC110050.053.1848371
GGGGAGC260150.052.9983831
ATCGGAA1173550.052.8894463
GGGGGGA191900.051.4762041
GAAGAGC1212600.051.1953247
GGGGGAT220900.049.676111
GGGGACC215100.049.633141
GGGGCGA221300.049.4224551
GGGGACT135650.048.7082981
GGGGGAA210550.048.501121
Produced by FastQC (version 0.11.5)