FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11998982
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTA1640281.3670159685213294TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA433350.075.372612
AGATCGG438450.074.563891
TCCGATC88850.072.220965
AGAGCAC443800.072.145059
TCGGAAG459100.070.308994
GAAGAGC470200.068.557547
CCGATCT93850.068.0642556
GGAAGAG481950.067.007856
ATCGGAA495000.065.750393
CGGAAGA502950.064.166785
AAGAGCA541400.059.608448
CGATCTG87600.059.262147
CTTCCGA125050.052.0650863
GATCTGG87500.049.800638
CGATCTA24900.045.4248667
ATCTGGG78700.042.3787279
TTCCGAT158550.041.2480354
TCTTCCG211500.032.017992
CTCTTCC215850.031.7766041
GATCTAG29800.028.4667598
Produced by FastQC (version 0.11.5)