FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11998982
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC1089130.9076853353059451Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC159650.076.772661
GGGGGCC165250.074.6537551
AGAGCGT420700.069.615679
GGGGACG140300.067.149121
AAGAGCG441050.066.814328
GATCGGA448050.066.454552
AGATCGG446050.066.436021
TCGGAAG460050.064.101884
GAAGAGC468600.062.9941377
GGGGAGC165700.060.051021
GGGGGCA105250.059.1915661
ATCGGAA500700.058.9720043
GGGGGCT79050.058.258681
GGGGCGA145750.056.626861
GGGGGGA138050.054.5317881
GGGGGTC69400.054.341631
GGGGACC148750.053.58331
CGGAAGA567500.052.6413575
GGGGAGA198900.052.069361
GGGGGAT155050.051.265781
Produced by FastQC (version 0.11.5)