FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14171178
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG556350.3925926270914104TruSeq Adapter, Index 6 (97% over 36bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205630.14510438017220587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG275750.062.6847081
GATCGGA281650.061.2413562
AGAGCAC284600.058.848339
TCGGAAG335800.050.696274
GAAGAGC342200.049.260297
GGAAGAG365350.046.338566
CCGATCT121950.044.70966
TCCGATC126550.043.256175
ATCGGAA397700.043.169963
AAGAGCA415200.040.704038
CGGAAGA420900.040.5672155
CGATCTG121200.037.1486477
GATCTGG109150.034.2086528
GATCTAG20450.031.9081548
CTTCCGA179600.031.0438483
ATCTGGG93550.030.0708039
CGATCTA37350.024.0704987
TTCCGAT261450.021.4640124
CTCTTCC296800.019.5432571
TCTTCCG333700.017.577312
Produced by FastQC (version 0.11.5)