FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13069623
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATC1531281.1716328772451967Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATA134130.10262729077954276Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC149900.078.086761
GGGGGCC154850.076.480451
AGAGCGT579250.068.0199669
AGATCGG583450.067.706321
GATCGGA589650.067.075282
AAGAGCG596500.066.210578
GGGGACG140550.065.3248441
GGGGGCA94950.064.999511
TCGGAAG640650.061.5902184
GGGGGCT79050.061.4679071
ATCGGAA658500.060.034163
GAAGAGC671800.058.994157
GGGGCGA140500.057.863511
GGGGAGC172550.057.1609541
CGGAAGA725800.055.013395
GGGGACC141550.054.821311
GGGGAGG128900.053.6748621
GGGGCCG132500.053.639621
GGGGGGA143950.052.3455051
GGGGGTC79800.051.9838751
Produced by FastQC (version 0.11.5)