FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10976797
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG209130.19052005790031462TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA117450.055.558072
AGATCGG122550.053.7197721
AGAGCAC120400.052.0882039
TCGGAAG143950.044.57544
GGAAGAG169600.037.6634066
GAAGAGC173350.036.6806837
ATCGGAA184100.035.4047553
CGGAAGA185950.034.663565
AAGAGCA211950.030.0003178
CCGATCT75350.029.252296
TCCGATC77150.028.663655
ACACGAC38250.025.589283
CGATCTG77100.024.262027
CTACACG42500.023.7130571
GATCTGG68350.021.8518568
TACACGA48400.020.6724242
CTTCCGA110800.020.4814323
ACGACGC51850.018.4583445
ATCTGGG65450.017.1700619
CGACGCT58100.016.7223856
Produced by FastQC (version 0.11.5)