FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005275883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10976797
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACACGTAATAGCAGATTGCACAAAATTTCTTCCCCAACGCACCAAT356870.32511305438189303No Hit
GGGGACACGTAATAGCAGATTGCACAAAATTTTCTTCCCCAACGCACCAA264920.241345448950181No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC242140.22059258269967094Illumina Single End PCR Primer 1 (96% over 33bp)
GGGACACGTAATAGCAGATTGCACAAAATTTCTTCCCCAACGCACCAATT176590.16087570900691703No Hit
GGGACACGTAATAGCAGATTGCACAAAATTTTCTTCCCCAACGCACCAAT137720.12546465057156472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)