FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12658702
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTA815960.6445842551629701TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG282700.061.912911
AGAGCAC282500.059.927749
GGAAGAG307750.055.5075576
GAAGAGC319000.053.29837
GATCGGA348400.050.008482
AAGAGCA343100.049.5120628
TCGGAAG378650.045.4771964
ATCGGAA413400.041.9692963
CGGAAGA416350.041.3423655
CCGATCT105050.041.067276
GATCTAG12550.041.017968
CGATCTA19900.039.3488357
TCCGATC119400.036.4351165
ATCTGGG69750.030.8724929
ACACGAC30600.030.0913543
TACACGA32450.028.1529452
GGCCCCA28350.028.132571
CGATCTG133950.026.3601237
GATCTGG119250.024.8062558
CGAATTA179850.024.4702139
Produced by FastQC (version 0.11.5)