FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15337249
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC5125923.3421378240647983Illumina Single End PCR Primer 1 (97% over 35bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATA652330.4253239938922554Illumina Single End PCR Primer 1 (97% over 35bp)
GGGTAAGTAAAACGGTTTTTTGAGTAACAAAAGTTTCCCAAAAACTATAT546000.3559960459662616No Hit
GGGGTAAGTAAAACGGTTTTTTGAGTAACAAAAGTTTCCCAAAAACTATA448330.29231448221255324No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATATTGTAGATC411740.26845753107353215Illumina Single End PCR Primer 1 (97% over 35bp)
GGGGTAAGTAAAAGGGTTTTTCCCGAAACAAAATATAATCAAAAACTCAA232290.1514548013141079No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTATATC208620.1360217859148013Illumina Single End PCR Primer 1 (97% over 35bp)
GGGTAAGTAAAAGGGTTTTTCCCGAAACAAAATATAATCAAAAACTCAAT188570.12294903734039918No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT1998850.075.90789
AAGAGCG2043450.074.524088
GAAGAGC2090550.073.077277
GATCGGA2105700.072.465592
AGATCGG2113750.072.187041
TCGGAAG2134050.071.407034
ATCGGAA2205550.069.198283
GGGGTAA193350.068.649281
CGGAAGA2296750.066.7238165
GGAAGAG2312800.066.2954566
GGGGGAC145550.059.7995761
GGGGGCC126100.055.3336141
GGGGACC116100.051.6657031
GGGGATC125750.048.2200131
GGGGACT96350.048.103671
GGGGACG153200.047.912721
GGGGAGC189000.044.0180361
GGGTAAG300050.043.8503151
GGGGGCT98900.042.096371
GGTAAGT287950.041.8995823
Produced by FastQC (version 0.11.5)