FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9317603
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG157420.1689490312046993TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA106850.044.580422
AGATCGG114850.041.9167981
GATCTAG7150.041.576018
AGAGCAC115800.039.069169
TCGGAAG149350.031.4090464
ATCGGAA165400.028.5362243
TCCGATC73400.028.350015
CCGATCT77000.027.2128776
GGAAGAG175500.026.8123556
GAAGAGC177100.025.9152167
AAGAGCA186900.024.55618
CGGAAGA205900.022.783235
GATCTGG63000.022.3267868
CGATCTG74900.022.1678377
CGATCTA20250.021.1249967
ATCTAGG9400.020.0541179
ACACGAC42100.019.287993
ATCTGGG57550.018.77159
AGTAGGG23200.018.7505631
CTTCCGA118150.018.347993
Produced by FastQC (version 0.11.5)