FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15358241
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTCAGTACGTGTAGATC1987561.2941325767709988Illumina Single End PCR Primer 1 (96% over 33bp)
GGGCAGACAGACAATCTGAAATAACTTCCGAATTTAGCAAAGCTAAAATC200280.13040555881366883No Hit
GGGGTTTGGGATAGCTATATAGCCAAAGGTTTTTGGATTGTTATTACATA157530.10257033992369308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGAC201350.088.866781
AGAGCGT575050.081.887439
GGGGACG159500.081.625471
AAGAGCG587850.080.399698
GGGGAGA185750.078.5245061
GAAGAGC607200.077.944687
GGGGGCC205450.077.8438641
AGATCGG631400.075.517411
GATCGGA634100.075.330262
CGGAAGA657450.072.74725
TCGGAAG654200.072.454674
GGGGAGT56350.072.208921
GGGGGAA137150.071.552351
GGGAGGC124200.071.0707551
GGGGAAC122950.070.2595141
GGGGAAG115900.070.152661
GGAAGAG689700.069.072676
GGAGGGA126100.067.871721
GGAGGGC102500.067.8604351
GGGGACT81450.067.143231
Produced by FastQC (version 0.11.5)