FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14278850
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTA2749241.925393151409252TruSeq Adapter, Index 7 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTG200970.14074662875511684Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA800650.078.0609052
AGATCGG816600.076.881941
TCCGATC137250.076.1785
AGAGCAC830150.073.450769
TCGGAAG847500.072.702464
GAAGAGC865500.070.5446557
CGGAAGA872650.070.51655
GGAAGAG873650.070.1538546
ATCGGAA885300.070.113243
CCGATCT150150.069.633486
AAGAGCA945000.064.6708768
CTTCCGA183450.057.9800263
CGATCTG168650.049.1391227
GATCTGG139300.048.1974838
CGATCTA45450.047.5389867
ATCTGGG110350.047.3060879
GATCTAG36700.045.4387328
TTCCGAT238250.044.6446344
CTCTTCC296050.038.4258841
TCTTCCG293900.037.7944682
Produced by FastQC (version 0.11.5)