FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14278850
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATC2155741.509743431718941Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT731600.070.755769
GATCGGA742500.070.274052
AGATCGG751050.069.4780351
AAGAGCG750700.069.23528
TCGGAAG774550.067.1496054
GGGGGCC98000.066.4654851
GGGGGAC109500.065.579341
GAAGAGC821550.063.4934847
ATCGGAA838750.062.1435283
CGGAAGA915850.057.232625
GGAAGAG958600.054.861676
GGGGACC101200.052.752521
GGGGACG138550.051.3580361
GGGGAGC135700.050.940011
GGGGGCA80550.050.4276121
GGGGGCT82000.042.459351
GGGGCGA150050.042.442841
GGGGGTC66300.042.120461
GGGGCCG120700.041.6458661
GGGGATC119550.040.8936841
Produced by FastQC (version 0.11.5)