FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15807420
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGG1000270.6327851097775602TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGT250110.15822316355230645TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA389400.074.291692
AGATCGG397600.072.851161
TCGGAAG424050.067.6406864
AGAGCAC421850.067.304889
ATCGGAA476850.060.439193
GGAAGAG509200.057.2140546
CGGAAGA513500.056.805195
GAAGAGC550550.052.8103647
AAGAGCA545900.052.329988
GATCTAG13350.032.5864758
CCGATCT124650.031.0038836
TCCGATC137700.028.0654285
CGATCTG125300.025.5765977
CGATCTA24150.024.3186477
ACACGAC51100.023.2694573
GATCTGG123900.022.4712568
ACGACGC59050.020.0145635
TAGATCG43550.019.9790236
CTACACG61300.019.042831
CTAGATC31650.019.0144085
Produced by FastQC (version 0.11.5)