FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15807420
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATC981340.6208097210044397Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG408550.065.8080441
GATCGGA415650.064.799532
GGGGGCC181150.060.8564831
GGGGGTC82000.059.7023661
GGGGACG178550.059.427311
AGAGCGT449000.059.4178739
AAGAGCG459200.058.5554588
TCGGAAG465050.057.5844354
GGGGGCT117200.055.756921
GGGGGGC189750.053.2440341
ATCGGAA518750.051.7099883
GGGGGAC263300.050.435821
GGGGCCG139050.050.1771161
GGGGACT107350.048.3740651
GGGGGAG191100.048.0479281
GGGGGGA209550.046.3441051
GGGCCGG131950.045.841441
GAAGAGC599250.045.1849067
GGGGCGA247800.042.849071
GGGGAGA298850.042.179181
Produced by FastQC (version 0.11.5)