FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005275897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005275897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10863603
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATC325830.29992811777087214Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGCC134850.075.303371
GGGGGAC148100.071.45581
GGGGGGC113350.066.038231
GGGGGCT73650.064.999261
GGGGACG131200.064.738151
GGGGGCA92500.061.6336821
GATCGGA132700.060.903352
GGGGGAT123150.060.2529751
GGGGGAG113050.058.6425551
AGATCGG139200.057.3700681
GGGGAGC152150.057.3496741
GGGGAGA175800.055.6583561
GGGGGGA119850.054.95221
GGGGAAG107600.054.3999561
GGGGGAA123250.054.2013321
GGGGACT81450.053.6986661
TCGGAAG149650.053.1098634
GGGGATC99100.052.405471
GGGGACC127800.052.101361
GGGCGGG156050.051.1753461
Produced by FastQC (version 0.11.5)