FastQCFastQC Report
Wed 11 May 2022
EGAF00005278237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278237
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences27021949
Sequences flagged as poor quality0
Sequence length90
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1702920.6301988061630935No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1561070.577704443154711No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1252970.4636860205753478No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1139730.42177934685614277No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA869250.32168294004255577No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT686750.25414525058869736No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC633520.23444644944004595No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT633470.23442794596348324No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA632360.23401716878379128No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC583790.2160428916507836No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA583540.2159503742679701No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG578840.2142110474710762No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT561530.20780514388506915No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT552020.20428578264284342No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546170.2021208758850074No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC540250.1999300642599836No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT536980.198719936892783No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA534250.1977096470724595No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG529520.19595921818962797No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT528070.19542261736930966No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG515590.1908041496192595No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC510140.18878727067392512No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT503620.18637441733014892No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA503360.18627819925202288No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT498630.18452777036919135No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT492210.18215192397854055No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG485720.17975017272070198No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG478340.17701905958004732No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTG455210.16845935132214185No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC445220.1647623567049142No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC409640.15159528278289625No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA407450.15078483050944994No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG404290.14961541079068721No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA398350.14741719777503834No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA395420.14633289404846408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT394750.14608494746252387No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCT388950.1439385441812506No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA387000.14321690859530525No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG375950.13912764027494834No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT374640.13864284918900557No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA363150.13439075027489691No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG353210.13071225913423196No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC349710.12941701577484288No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC344220.12738533404825833No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG326790.12093502211850077No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT324040.11991733090755223No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTT320100.11845925695441138No Hit
GGTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT315780.11686055657939404No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC314110.11624254046219981No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC310500.1149065894543728No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT303050.11214957144653037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302440.11192382903246541No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC299510.11083952530589115No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT291650.10793077879023456No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA288340.10670584864178376No Hit
GACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT276040.10215399340735934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)