FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278251
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14438047
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT339670.23526035065545917No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC322760.22354824028485293No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA312440.21640045914797204No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294750.20414810950539225No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA278500.19289312467260983No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT223980.15513178479056067No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA208490.14440318694072682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207430.14366901562240378No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG185670.12859772516324403No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC176360.12214948462212374No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA171930.11908120260309445No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT162200.11234206399244995No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT162150.11230743326988754No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC161660.11196805218877594No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155850.10794396222702421No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC155750.10787470078189938No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT151740.1050973168323943No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG148490.1028463198658378No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA147150.1019182165011653No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG144630.10017282808401995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA455800.038.788961
GGTATCA250100.038.7382051
ATCAACG596600.029.4098033
TCAACGC605550.028.9473744
AACGCAG610700.028.7110446
CAACGCA611300.028.659955
ACGCAGA641100.027.2775337
CGCAGAG650400.026.8810378
GTATTAG154150.025.4844151
GCAGAGT683400.025.290079
ATTAGAG134350.024.6748333
TATTAGA141550.024.3399052
TATCAAC726900.024.1957682
GAGGCAC141700.022.500727
AGAGTAC655850.021.61026410-11
GTACATG158450.021.2320081
TACATGC152200.020.7871632
CATGCTA149300.020.76884
CAGAGTA674650.019.76607110-11
TTAGAGG191850.019.2723984
Produced by FastQC (version 0.11.5)