FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278253
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences10592049
Sequences flagged as poor quality0
Sequence length90
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG359300.3392167086840327No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC341510.322421091518742No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA318220.30043290018767854No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT278630.26305580723805183No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT250650.23663976630017478No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC231330.2183996694124055No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC225010.21243292964373559No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG209410.1977049011008163No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT206370.19483482374373456No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA206140.19461767973316588No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT186400.1759810590000103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184850.17451769718965612No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA183190.17295048389598652No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA177410.16749356049995615No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG167270.1579203419470586No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC166640.15732555617897914No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG159050.15015980383021266No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC157190.14840376965778765No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT156600.1478467480654593No Hit
GTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACT148670.14036000022280862No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA146940.13872669962157463No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG137900.13019199590183164No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA130980.12365879349689564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129440.12220487273047924No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC124480.11752211493734593No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG120860.11410445703187362No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG118470.11184804753074688No Hit
GTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAAC117530.11096058940059661No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG112530.10624006743171222No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC112400.10611733386052123No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG111940.10568304583938387No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT109570.10344551842613266No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC108850.1027657632626133No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC108470.10240700359297808No Hit
AGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACAC106410.1004621485417977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA134650.070.620291
GTATCAA353150.049.4284131
TATAACG3600.036.261112
ATCAACG526450.033.354613
TCAACGC535200.032.7788054
CAACGCA545250.032.204715
AACGCAG555800.031.7035186
GTGGTAT24400.031.4312481
TGGTATC22600.030.8660642
TATCAAC581750.030.026442
ACGCAGA589300.029.6797247
CGCAGAG591150.029.6351538
CTATACG705.012233E-429.1415564
ATAACGC4650.028.9535453
TAACGCA5050.028.2759634
GTATTAG90700.028.1103531
GCAGAGT604350.027.6844719
ATTAGAG90100.027.0780353
TATTAGA91150.026.8079592
GTACATG97600.025.9558561
Produced by FastQC (version 0.11.5)