FastQCFastQC Report
Thu 12 May 2022
EGAF00005278257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70961871
Sequences flagged as poor quality0
Sequence length90
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3778300.5324408653204761Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1797060.25324304090009125Illumina Single End PCR Primer 1 (100% over 50bp)
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1313840.18514731664840123No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1047080.14755529768937461No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG1006670.14186069022898226No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC793210.1117797471828216No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG739230.10417284516074836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)