FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278259
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences12220113
Sequences flagged as poor quality0
Sequence length90
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA362550.2966830175792973No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT342290.2801037928209011No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC340320.278491696435213No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA310720.2542693344979707No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT193250.15814092717473235No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA184440.15093150120624907No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT159330.13038340971151413No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA154290.12625906159787556No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG141160.1155144801034164No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA140710.11514623473612723No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA139650.11427881231540167No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT139150.11386965079619149No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG134780.11029357911829457No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC134550.11010536481945787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134120.10975348591293714No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT132340.10829687090454891No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT129520.10598919993620352No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA127770.10455713461896793No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC127240.10412342340860514No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT123800.10130839215643914No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA174200.030.1403982
ATTAGAG179350.030.0055183
GTATTAG179850.029.9647711
GAGGCAC190700.027.7489787
GTACTGG77650.027.4250641
GTATCAA782250.026.0709081
CATGCTA184600.025.8369064
GTACATG187950.025.6996941
AGAGGCA208550.025.5304246
GTATAGA42850.025.0393771
TATAGAC39050.024.86422
ATGCTAA194750.024.5741355
GTGGGTT211500.024.42358884
TAGACCC39800.023.782784
ATAGACC41250.023.6391413
TGCTAAG204500.023.24296
TCACTAT82400.023.0599179
TCTATAC37050.022.9051343
AGGCACC239400.022.6153188
TGGTTCA82850.022.6036135
Produced by FastQC (version 0.11.5)