FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278265
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences11894068
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144450.12144709446759511No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC140740.11832789252592132No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA126740.1065573191611146No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA119930.10083177597437648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA303100.038.653961
GGTATCA163700.035.077811
TCAACGC400100.028.6881164
ATCAACG405600.028.3716073
CAACGCA405150.028.2677175
AACGCAG410050.028.0795926
TATCAAC424850.027.0860882
ACGCAGA429300.026.6737167
CGCAGAG435650.026.3234928
GTATTAG80100.024.7545571
GCAGAGT457950.024.5061349
AGAGTAC441700.020.93853610-11
TATTAGA85750.020.2841172
ATTAGAG82400.019.987053
CAGAGTA447650.018.72743810-11
AGTACGG359400.017.50553912-13
GTATATA51150.016.543111
GGCACCG95200.016.2796789
CCTATAC74950.016.2000393
TACGGGG137650.016.18533514-15
Produced by FastQC (version 0.11.5)