FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005278281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5245994
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA879101.6757548712407984No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT562511.0722658089201018No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG388960.7414419459877385No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC248400.4735041633673237No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT224510.4279646526473343No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG206540.39370994324431174No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT206360.3933668242853499No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT167890.3200346778894524No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC162970.3106560930111624No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG161620.30808270081894873No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC161070.30703428177767644No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG150510.28690463618524914No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT138930.2648306498253715No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC138720.26443034437324936No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT137500.2621047603180637No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA136520.2602366682081603No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC135680.2586354463996718No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT135280.2578729598242011No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA128900.24571129894544294No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG125580.23938266036903588No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA122930.23433118680654227No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA120350.2294131483947561No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC118820.22649663724358055No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT117380.22375168557188593No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG115450.22007268784523965No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT114050.21740398483109208No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA107230.2044035887193161No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA106670.2033361075136571No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC105330.20078177748583015No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT104980.20011460173229326No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG104970.20009553956790652No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA102700.1957684282521101No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA101850.19414814427923477No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA101810.1940718956216877No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG98980.18867730310023229No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT94350.17985152098915858No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC94160.17948933986580998No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT93320.17788811805732146No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC90400.1723219660563851No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA90020.17159760380968794No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC86390.16467803813729104No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC85740.1634389974521511No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA85110.1622380810957847No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG85010.162047459451917No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT84140.16038905115026816No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA81980.15627162364272623No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT79240.15104859060075174No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC77820.14834176325783066No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC77640.1479986442988688No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA76550.14592086838071108No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG76510.145844619723164No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC76150.14515838180524035No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG75480.14388121679132687No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA75320.14357622216113858No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA75250.1434427870104312No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT73380.13987816227010555No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG71000.1353413671460547No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA70100.13362577235124554No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG70020.1334732750361514No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT68190.1299848989533728No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG68050.12971802865195803No Hit
GACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG67850.1293367853642227No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT67590.12884116909016669No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTAT66540.12683964182955604No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT64580.12310345760974946No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC64490.12293189813026854No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT63900.12180723043144921No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC63550.12114005467791233No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC62790.11969133018451794No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGC61810.11782323807461464No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA61330.11690825418404976No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG61080.11643170007438056No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG58310.11115148053924577No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG56910.10848277752509819No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC56730.10813965856613637No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC56670.10802528557981576No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAAC56310.1073390476618921No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA54710.10428910136000917No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACA54640.1041556662093018No Hit
GGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCA54540.10396504456543412No Hit
CCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTGGGGT54340.10358380127769874No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC53650.10226851193501174No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG53380.10175383349656901No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)