FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5332272
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTATCCAGAATCTCGTATGCCGTCTTCTGCTTG521710.9784009517894061RNA PCR Primer, Index 46 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410440.7697281759070055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404080.7578008023596695No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA280930.5268485928699811No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCCAGAATCTCGTATGCCGT278980.5231916151314112No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265700.49828665904515No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT180100.3377547131879244No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT171480.32158899621024584No Hit
ATACACATCTCCGAGCCCACGAGACTATCCAGAATCTCGTATGCCGTCTT162930.305554555356516RNA PCR Primer, Index 46 (95% over 23bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139790.2621584195254856No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA139290.26122073292585224No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135550.254206837160595No Hit
CATCTCCGAGCCCACGAGACTATCCAGAATCTCGTATGCCGTCTTCTGCT131710.247005404075411RNA PCR Primer, Index 46 (96% over 28bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG116210.21793711948677788No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG106400.199539708401972No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT103610.19430741717601802No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC99240.18611203629522274No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT98160.18408663324001476No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT97070.1820424764528141No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC93820.17594751355519747No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91230.17109029696909683No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG91200.17103403577311885No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC89150.1671895207146222No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA77270.14491008710733436No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC74960.1405779750170284No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA73450.13774616148613572No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA71490.13407043001557312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69670.1306572507929078No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG68700.12883813878961914No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA66420.12456228789529115No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA65170.12221807139620786No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA63410.11891741456549854No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT63050.11824228021376254No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA62980.11811100408981388No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG61730.11576678759073054No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT60660.11376013826751524No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT60220.1129349740598379No Hit
ACACATCTCCGAGCCCACGAGACTATCCAGAATCTCGTATGCCGTCTTCT59540.11165972028433657RNA PCR Primer, Index 46 (96% over 25bp)
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC58350.10942802617720927No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCCAGAAACTCGTATGCCGT57400.10764642163790594No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG55370.10383941404339464No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC55360.10382066031140197No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA55000.10314552595966599No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG54470.10215157816405465No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT54450.10211407070006931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.078.953911
GTATCAA144550.051.9187361
AGTACGG158250.048.7195172
GAGTACG158950.048.6693271
GTACTGG35950.047.629911
TACTCCG68850.047.3660245
TAATACT45800.047.3184134
TCCGGTC68400.047.1993988
CCGGTCT69550.046.4189649
ACTCCGG69700.046.2516486
CTCCGGT70800.045.40037
TACGGGT20000.045.3651164
ACGTGTC64550.044.9912538
TACCTTC28850.044.9571887
CGTGTCC65300.044.186659
CACGTGT66550.043.2154667
ATGCGGA24100.042.1184736
ACCACGT68950.041.5044335
TATGCGG25400.041.0705645
TACCACG70150.040.744654
Produced by FastQC (version 0.11.5)