EGAF00005278303 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005278303
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5332272
Sequences flagged as poor quality	0
Sequence length	100
%GC	47

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA	24884	0.46666786690551415	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT	15715	0.29471489826475467	No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT	15514	0.2909453981342287	No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG	13709	0.2570949118874656	No Hit
CATCTGACGCTGCCGACGAGGCAACAGGTGTAGATCTCGGTGGTCGCCGT	13569	0.2544693894084923	Illumina RNA PCR Primer (96% over 25bp)
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA	13243	0.248355672778883	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT	10813	0.2027841040367033	No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG	9784	0.18348651381624945	No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC	9649	0.18095475999723945	No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT	9490	0.17797291661040546	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT	9462	0.1774478121146108	No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC	8897	0.1668519535387542	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC	8060	0.1511550798608923	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC	7758	0.14549145279910702	No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG	7592	0.1423783332883244	No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA	7224	0.1354769599150231	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7112	0.13337654193184442	No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA	6926	0.12988834778120847	No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG	6922	0.1298133328532378	No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC	6794	0.1274128551581765	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA	6584	0.1234745714397165	No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA	6554	0.1229119594799365	No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG	6449	0.12094281762070652	No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT	6399	0.1200051310210732	No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA	6275	0.11767966825398253	No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATT	6259	0.11737960854209988	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6192	0.11612310849859121	No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT	6151	0.11535420548689189	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT	5899	0.11062826502473992	No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA	5803	0.10882790675344393	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	5791	0.10860286196953194	No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA	5657	0.10608986188251462	No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG	5484	0.1028454662477833	No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT	5453	0.10226410055601065	No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT	5423	0.10170148859623064	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA	5396	0.10119513783242864	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4050	61.59621	1
TAATACT	4960	44.06634	4
GAGTACG	15980	43.989403	1
TACTCCG	6745	43.964447	5
AGTACGG	16000	43.89282	2
GTATCAA	7185	43.812023	1
GTACTGG	3665	43.397396	1
TCCGGTC	6820	42.930508	8
ACTCCGG	7010	42.100147	6
CTCCGGT	7035	41.614193	7
CCGGTCT	7130	41.19386	9
ACGTGTC	5960	40.214832	8
CGTGTCC	5960	39.818703	9
TACCTTC	2750	39.130943	7
TACGGGT	2000	38.543358	4
TAGTTCG	5250	38.2196	7
ACCACGT	6245	38.002586	5
TATGCGG	2960	37.94551	5
TGGTTCA	3880	37.91114	5
AGTTCGC	5335	37.614544	8

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content