FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278305
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences15556456
Sequences flagged as poor quality0
Sequence length90
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT432660.2781224721106144No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC378710.24344233673787913No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA378500.2433073445519982No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT373680.24020895247606525No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC347510.22338635483557437No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG344680.22156717442584611No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA330140.21222057260342586No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT322770.20748298969893914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312210.2006948112089283No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT276250.17757900642665655No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA274520.17646692794297106No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC257370.1654425660960311No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT251330.1615599337021234No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT244410.15711161976738147No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC234790.15092769201417083No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA228630.1469679212283312No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218230.14028259392756293No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA216970.13947264081227756No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG216010.13885553367682202No Hit
GTGCTGTGTTTTTAATAAACAGTTGCAGCCAGCTGGTATCTTCGACTGAT214580.1379363011729664No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA206290.13260732393033478No Hit
GGATACCACGTGTCCCGCCCTACTCTTCGAGTTCACAACGTGTGCATTTT188360.12108156253583721No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT185030.11894097215972585No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT183310.11783532187536802No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT178290.1146083658128818No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGGCGTGCTA178150.11451837102229455No Hit
CTATCATGTCATGAATACATAGTGGCATGAGGCGAACCGGGGGAACTGAA176110.11320701835945154No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC174430.11212708087240436No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG174350.11207565527778306No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC168830.10852728924891376No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG168440.10827658947513495No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT163840.10531961778441053No Hit
GTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACT162260.10430396229063998No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA159670.10263905866477559No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC156680.10071702706580471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA912900.039.3733141
GGTATCA555650.036.864161
ATCAACG1261000.028.4713753
TCAACGC1270600.028.2213884
AACGCAG1275500.028.0112366
CAACGCA1280250.027.9716855
GTATTAG142100.027.6652531
TATTAGA137650.027.1863922
TATCAAC1327100.026.9830952
ATTAGAG139750.026.8353823
GTATAGA62400.026.6540681
ACGCAGA1346950.026.3580787
CGCAGAG1345450.026.2844148
TAGACCC58550.026.2503074
GTACTGG102000.026.1472761
TATAGAC60500.025.6859972
GACCCGA59100.025.3578766
GCAGAGT1361700.025.1833489
GAGGCAC145900.024.7452627
AGACCCG61350.024.4354255
Produced by FastQC (version 0.11.5)