FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283437
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG42030.18406463589755268No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA29600.12962915114364879No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT25510.11171755559711086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACG37150.044.7741241
GTACGGG67200.044.371421
AGTACGG40700.041.812522
ATAATAC22550.041.6878053
TACGGGA36150.039.7928732
GTATAAT18750.039.0637251
TAATACT24400.038.13764
CTATACT19250.037.598284
TATACTC18950.035.463075
ACTATAC19700.034.118933
CTAAGTT13800.034.0534329
GTATCAA20300.033.9861
ACTAAGT14300.033.8493778
GTATAGT17550.033.1185461
TACGGGG37950.032.4550062
TACTAAG16300.030.8493257
TATACTG10150.030.095175
CCGGTCT11100.030.0590579
TACTCCG11650.029.8507545
ATAGTAC11050.029.7756563
Produced by FastQC (version 0.11.5)