FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005278322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278322
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22836980
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1796890.7868334604663139No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1312440.5746994567582929No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1282550.5616110361352509No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1187980.5202001315410356No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT1055380.4621364120825083No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA802460.35138621656628855No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA638070.27940209257090914No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC633150.2772476921204117No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA597460.2616195311288971No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT587850.2574114440701003No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG565630.24768161114122797No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT557480.24411283803725364No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA556320.24360488996355908No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT554260.24270284424648092No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT550550.24107828618319935No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG531300.23264897547749305No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC531270.23263583888938028No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC523550.22925535688168927No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT522190.22865983155390948No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT513710.22494655598069446No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT500180.21902195474182662No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA495060.21677997703724397No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC495020.21676246158642692No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG491380.2151685555620752No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA481670.21091667987623583No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCT473790.20746613606527656No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG463830.20310478881183067No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT447120.19578770923300717No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC426690.18684169272819787No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC426190.18662274959298472No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG420690.18421437510564007No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTG413960.18126740050567108No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT413460.1810484573704579No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG410420.17971728310836196No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG400510.17537783016843733No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG395890.17335479559906783No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA392900.1720455156504932No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC383930.1681176758047693No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA378010.1655253890838456No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTT372320.16303381620511995No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG351300.1538294468007591No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343510.15041831275413825No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC334570.14650360949652713No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC329700.14437110335955103No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC326140.14281222823683343No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA317890.13919966650581644No Hit
GGTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT316380.13853845823747274No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC313240.13716349534833416No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT312830.13698396197745938No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA298200.13057768584112261No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC297710.1303631215686137No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT288340.1262601272147193No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG279220.12226660442843143No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG269930.1181986409761711Clontech Universal Primer Mix Long (96% over 25bp)
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT265210.11613181777975898No Hit
ACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGC264670.11589535919372877No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT260470.11405623685793831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259060.11343881721663723No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC252820.11070640688917713No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTG252300.11047870602855545No Hit
GCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGA250850.10984377093643731No Hit
CTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATT249360.10919132039350211No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGAT246280.10784263068058912No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA246220.10781635750436354No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC244650.10712887605979424No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG240990.10552621231003399No Hit
GTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTT240090.10513211466665032No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG239390.10482559427735191No Hit
CTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCC236740.10366519566072223No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)