FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4941935
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA843091.7059916813960523No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT570611.1546287031294422No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG536771.0861535006024967No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT448330.9071952585373948No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG373090.7549472018551437No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG349810.7078401476344792No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC315350.6381103757940969No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302290.6116834802562154No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT276220.5589308641250846No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260320.526757231732105No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC249690.5052474385033393No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC204150.4130972989325032No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC191470.38743933297382505No Hit
GGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGC170350.34470303636126337No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA168680.34132379321055417No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158070.3198544699596413No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT156420.31651569678678493No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC153360.3103237901753058No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT152410.3084014662272976No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA132170.26744584864025933No Hit
GGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGTCTTGGTGGTG129840.2627310962204076No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC129460.2619621666412043No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG127730.2584615135569367No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT127710.2584210435790839No Hit
GACCAACGCCGTGGCGCACGTGGACGACATGCCCAACGCGCTGTCCGCCC127200.25738905914383736No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC126780.2565391896089285No Hit
CCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGG123700.25030681301959656No Hit
GGATGTTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGAC122200.2472715646806362No Hit
GCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC121470.24579441048900885No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA121060.24496477594302635No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA119370.2415450628144644No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC119310.24142365288090598No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT119140.24107965806915715No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG118750.24029049350102744No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT117910.23859075443120964No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA115090.23288448755396418No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG112110.22685446085389632No Hit
GGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCA110710.2240215624042No Hit
GTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAAC109610.22179571362229575No Hit
CGTCAAGGCCGCCTGGGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTG105790.21406594785241No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT105190.21285184851682593No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA104320.2110914044802289No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC100260.20287599897610958No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA97420.1971292621210113No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA96470.19520693817300308No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA95590.19342625914747968No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG95570.19338578916962687No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG95290.19281920947968761No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG94340.1908968855316794No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT93940.1900874859746233No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG93350.18889362162796555No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT91550.18525132362121316No Hit
GAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTAC91290.1847252139091267No Hit
CCCGAAGCTTGTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGC90000.1821149003376208No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT89320.18073892109062542No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG89210.180516336212435No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA88760.1796057617107469No Hit
GGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCA87200.17644910343822814No Hit
GCTCTGCCCAGGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACC86720.17547782396976083No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA83780.16952873722539857No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA82850.16764688325524316No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG80290.16246672609008414No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA80020.16192038138907128No Hit
GTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTG77390.15659857930142745No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG77010.15582964972222418No Hit
AGATTGAAGAGTTCAAATCTGACCAAGATGTTGATGTTGGATAAGAGAAT76270.1543322605416704No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC76020.15382638581851038No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA74320.15038643770102197No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG72660.1470274295392392No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT72600.14690601960568075No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT71850.14538839543620058No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC71740.14516581055801017No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG71610.14490275570196695No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT71360.14439688097880687No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71130.14393147623349964No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT70950.1435672464328244No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69590.1408152879388337No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG69340.1403094132156736No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA69230.14008682833748318No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC68670.13895366895760466No Hit
GCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGG68410.1384275592455182No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA68090.13778003959987334No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC67860.13731463485456608No Hit
GACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG65120.13177024788873185No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA64330.13017168376354607No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT63630.1287552345386979No Hit
CAGCAATGCAGCTCAAAACGCTCAGCCTAGCCACACCCCCACGGGAAACA62230.12592233608900158No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA61110.12365601732924451No Hit
GGTTAAGGGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGG60890.12321084757286366No Hit
CCCCTATACTGGGCCCTATTCAGTGGCAGCTTCTTGTTCCATAGGATTAA60850.12312990761715804No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT60490.12240144801580757No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG60190.12179439834801552No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA59530.12045888907887296No Hit
AGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGC59340.1200744242892713No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC57540.1164321262825189No Hit
GGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGG57390.11612860144862285No Hit
GAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA56990.11531920189156676No Hit
GGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGAT56920.11517755696908195No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG56900.11513708699122914No Hit
GCCCAAGATAGTTAAGTGGGATCGAGACATGTAAGCAGCATCATGGAGGT55470.11224348357475361No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC55160.11161619891803515No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG54710.11070562441634704No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG54690.11066515443849423No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG54330.10993669483714374No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC54230.10973434494787973No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG53850.10896541536867645No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG53790.10884400543511802No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA53630.1085202456122956No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT53240.1077310810441659No Hit
GGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCT53090.10742755621026985No Hit
GTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGA52800.1068407415314042No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT52760.10675980157569859No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG52420.1060718119522009No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACA52350.1059301670297161No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAAC51780.10477677266091116No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC51710.10463512773842634No Hit
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAG51220.10364361328103262No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC50710.10261162884578612No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC49760.10068930489777789No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA49690.10054765997529308No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG49570.10030484010817625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44300.082.667491
ATAATAC61950.069.354213
TACTAAG57850.068.077437
ACTAAGT59200.066.366218
GTATAAT62400.066.327211
AATACTA64700.064.860295
CCGGTCT139900.061.0715079
TCCGGTC140000.060.860048
ATACCTT93550.059.58046
TACCTTC93800.059.2211847
TAATACT94550.059.0136874
CCCCTAT106800.058.1515581
CTCCGGT146700.058.0804757
ACTCCGG149400.057.156656
TATACTG14450.056.911755
TACTCCG153350.056.0481955
CCTTCTG100400.055.1877339
GTATCAA110200.054.9013671
GGATTAC158250.054.394861
TATACCT103650.053.7255365
Produced by FastQC (version 0.11.5)