FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278361
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences16368402
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT554070.33849975092253964No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA502560.3070305824600349No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA445740.2723173587745462No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC420060.25662859453231907No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT274600.16776225315091847No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT258160.15771851155659544No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA255680.15620339725282897No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG226260.13822974289121198No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC219000.133794367953573No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT218460.13346446403259157No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA208790.12755674011427626No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG204720.12507024204317563No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC201120.12287088256996621No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA198530.12128856561562944No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG198300.12114805098261883No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT196720.1201827765471547No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG194110.11858824092907787No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193320.11810560371134579No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT189760.11593068156561649No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC188460.11513646842251307No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG186830.11414064732769881No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC183260.11195961585009948No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT181610.11095157609154517No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT179010.10936314980533836No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC177210.10826347006873366No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA173880.10622906255601493No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT172460.10536153743047122No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT170480.10415188972020605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166450.067.785891
GTATCAA436150.048.8665961
ATCAACG630200.033.60223
TCAACGC643100.032.940864
CAACGCA660350.032.1050835
AACGCAG663750.032.06366
ACGCAGA701200.030.258017
CGCAGAG703700.030.1563138
GCAGAGT739800.027.7043029
GTATTAG246150.026.64061
TATTAGA240550.026.1074942
ATTAGAG240350.026.029183
CATGCTA281550.025.4090774
GTACATG293750.024.9908641
TAAGACT294300.024.3434589
GAGGCAC254750.023.9330967
AGAGTAC714800.023.81059810-11
ATGCTAA307050.023.4318035
AGTACGG469000.023.39886312-13
TACATGC309400.023.2258172
Produced by FastQC (version 0.11.5)