FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278410
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19265379
Sequences flagged as poor quality0
Sequence length90
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC883730.4587140486569197No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA811890.4214243592093361No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT790530.41033711301500997No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG784850.4073888190831854No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT684130.35510850837660657No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT668640.34706817862238787No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA622990.32337282334284734No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC616810.32016499649448893No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC580360.3012450468791712No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC579140.3006117865628286No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA545230.28301026416350283No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT507280.2633117157985836No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC501480.2603011339667909No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA494110.2564756187770819No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT491060.25489246798622545No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT481850.25011187166367194No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG466540.2421649737594054No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG446350.23168503458976852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417370.21664250674746652No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA413390.2145766247318571No Hit
GAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGG393220.20410706687888155No Hit
GTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACT379720.19709967813246757No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG366370.19017014926101375No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA365670.18980680317786636No Hit
GTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAA344270.17869879435021754No Hit
TAGTGATACTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTG343570.17833544826707015No Hit
ATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGAC336450.17463969953562813No Hit
GGGTAAGGTCGGCGCGCACGCTGGCGAGTATGGTGCGGAGGCCCTGGAGA323070.1676945986891823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321870.16707171968950105No Hit
GCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACACC316760.16441929328252508No Hit
ACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCG303550.15756243362770075No Hit
TTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTT301440.1564672047199279No Hit
GTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAAC301130.15630629431167692No Hit
GGTTAAGGGCCACGGCAAGAAGGTGGCCGACGCGCTGACCAACGCCGTGG300320.15588585098689206No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA293900.15255344833859744No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA291660.1513907408725258No Hit
TATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTA283360.14708249445806387No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC282900.14684372417485272No Hit
GTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGC280720.14571216065876513No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG278400.14450792792604808No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT277750.14417053513455408No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG275370.14293515845185295No Hit
TGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCT269500.13988824201174554No Hit
ACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT268720.13948337066195274No Hit
CTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAAC266270.13821165937093685No Hit
GTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAG266090.13811822752098465No Hit
GACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCG265590.13785869460445083No Hit
CTGATAGGCAGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGG263010.13651950475513616No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA260510.13522184017246688No Hit
AGCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACAC256610.13319748342350285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255830.13279261207371004No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC255170.13245002862388539No Hit
GGCGAGTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCAC253060.13135479971611252No Hit
CCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGG251960.13078382729973806No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT251240.1304100998999293No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA251090.13033224002496915No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA250010.12977164892525603No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG248420.1289463342506784No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG243790.1265430594435749No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC241590.12540111461082598No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT240130.12464327849454712No Hit
TTCCTGTCCTTCCCCACCACCAAGACCTACTTCCCGCACTTCGACCTGAG240020.1245861812529097No Hit
GTATCAACGCAGAGTACGGGACTCTTCTGGTCCCCACAGACTCAGAGAGA238860.12398406488655116No Hit
AAATAAATGTTTTTTATTAGGCAGAATCCAGATGCTCAAGGCCCTTCATA238180.12363110012006513No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC238020.12354804958677429No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT236310.12266044701222852No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT234330.12163269666275447No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT233760.12133682913790587No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT233400.1211499654380015No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG232610.12073990342987802No Hit
GACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG229630.11919308724733628No Hit
GGTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCAC227320.11799404517294988No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC225080.11683133770687823No Hit
GGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCA222710.11560115168250777No Hit
GGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGC222020.11524299625769108No Hit
AGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGGGGTGA219340.11385189982506963No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA215040.11161991674287851No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG214030.11109566025148013No Hit
AAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCAT213780.1109658937932132No Hit
GCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCCTGCACTGGTGG211830.10995371541873118No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA211630.10984990225211765No Hit
GTATTTGGAGGTCAGCACGGTGCTCACAGAAGCCAGGAACTTGTCCAGGG209810.10890520243593442No Hit
GCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGTGCA209140.10855742832777907No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT205970.10691198963695446No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG205880.10686527371197836No Hit
GCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCTTGTCACAGTGCA205510.10667321935374331No Hit
GAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA205110.10646559302051622No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC201720.10470595984641673No Hit
GTATGGTGCGGAGGCCCTGGAGAGGATGTTCCTGTCCTTCCCCACCACCA201220.10444642692988289No Hit
ACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGA200810.10423360993832512No Hit
GGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGAT200790.10422322862166375No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCT200570.10410903413838887No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA199640.1036263029136359No Hit
GTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAA199320.10346020184705425No Hit
GCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACC199020.10330448209713394No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG198380.10297227996397061No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTG198350.10295670798897856No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT197090.10230268503931327No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG196370.10192895763950452No Hit
CTAAAGGCACCGAGCACTTTCTTGCCATGAGCCTTCACCTTAGGGTTGCC196000.10173690328126948No Hit
AACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTG194450.10093235124001454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA265000.065.14821
GTATCAA612200.049.770661
ATCAACG930050.032.6949843
TCAACGC953750.031.8825364
CAACGCA972400.031.4035855
AACGCAG986250.030.9541456
ACGCAGA1051300.028.9509837
CGCAGAG1054800.028.7832818
TATCAAC1078150.028.4234772
GTGGGTT259950.027.65050984
GCAGAGT1099900.027.2321939
TATTAGA229400.025.659252
ATTAGAG239250.024.8679663
GTATTAG244600.024.778851
CATGCTA186750.024.7263584
GTACATG193200.024.5360971
AGAGTAC1081650.024.26853410-11
TATTAGG123900.023.9246082
ATGCTAA197500.023.4242325
GAGGCAC244100.023.2864287
Produced by FastQC (version 0.11.5)