FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005278413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45672172
Sequences flagged as poor quality0
Sequence length100
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGG5597081.225490217544285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4994721.0936024676032485No Hit
CGCCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGG3413420.7473741340788435No Hit
GTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCC2568220.5623161517258255No Hit
GTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTC1472480.3224020088206009No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1469490.3217473432180979No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1442460.3158290785907883No Hit
AACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCC1388990.30412173084301747No Hit
CCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCG1342370.2939142022849275No Hit
GGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAG1152350.25230899901147685No Hit
GAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGA1110560.24315900719589162No Hit
GGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGG1047470.22934534403137208No Hit
GTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAG1039290.22755431907201612No Hit
GGGCACGCGCCCTCCCGCGCGCGCCGGGACCGGGGTCCGGTGCGGAGTGC1031960.22594940306320446No Hit
GTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCC1027940.22506921720298304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1015400.2223235628031879No Hit
GGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGG974520.2133728170405384No Hit
GGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCC949350.2078618025873611No Hit
GACCCCGGTCCCGGCGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGG944090.20671011661105146No Hit
GCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACGCACCGCACGTTCGTGGG937020.2051621280459357No Hit
GTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCCCCGTTTCC925960.20274052217179425No Hit
GGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGG913350.19997954115254252No Hit
GGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACGCA910690.19939712961319203No Hit
GCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCC909380.19911030287764728No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830260.18178684385756824No Hit
GTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTC828420.1813839727175664No Hit
CTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACG741680.16239210169378412No Hit
CCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGC702100.1537259931496142No Hit
CCGGGACCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCG668010.14626192947425404No Hit
CCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGGCC666830.1460035664605572No Hit
CCGCACCGGACCCCGGTCCCGGCGCGGAGTGCCCTTCGTCCTGGGAAACG658640.14421035198413598No Hit
CCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCGG620940.13595587264822878No Hit
GTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGC618340.13538659821126967No Hit
CCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGC576110.12614026764481445No Hit
AATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTT570200.12484626305926506No Hit
GCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCG550860.12061173705511531No Hit
CCGGGGTCCGGTGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCG532400.11656988855270557No Hit
CCTTTCCGGCCGCGCCCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGA519340.11371037926551862No Hit
GTCCTGGGAAACGGGGCGCGGCTGGAAAGGCGGCCGCCCCCTCGCCCGTC519150.11366877844127929No Hit
GGACCCCGGTCCCGGCGCGGAGTGCCCTTCGTCCTGGGAAACGGGGCGCG518060.11343012108116952No Hit
CCCCGTTTCCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCG513940.11252804005029582No Hit
AACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAGCCGCGCC500980.10969042593376116No Hit
TGACGGGCGAGGGGGCGGCCGCCTTTCCGGCCGCGCCCCGTTTCCCAGGA499980.10947147422723841No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC490800.10746149756135968No Hit
CCCTTCGTCCTGGGAAACGGGGCGCGGCTGGAAAGGCGGCCGCCCCCTCG490060.10729947329853286No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGG479780.10504864975547912No Hit
TGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGG477460.10454068179634637No Hit
GTCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAACCTGTCTCTTAT467720.10240809217481489No Hit
GTCCCGGCGCGCGGCGGGGCACGCGCCCTCCCGCGCGCCGGGACCGGGGT458480.1003849784065448No Hit
GGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCCCGTCACGCACCGCACGT457570.1001857323536091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)