FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278424
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences21321558
Sequences flagged as poor quality0
Sequence length90
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA646060.3030078758784888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612120.2870897145508785No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT500490.23473425347247137No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG493650.23152623274528064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT486380.22811653820044483No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC462670.21699633769727333No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC428940.20117666823409433No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA425970.19978371186570887No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT410420.19249062380901058No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT380320.17837345657385825No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA378950.1777309144106636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372850.17486996025337362No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT357650.1677410253040608No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT326600.1531782996345764No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC311370.1460352944189163No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG309410.14511603701755751No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA298200.13985844749243934No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC286470.13435697335063412No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT262990.12334464488945882No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC256920.12049776099851614No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC236150.1107564465973828No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA233880.10969179644376831No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT230040.10789080235131035No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT229720.10774071951027218No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA223200.10468278162411959No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA221770.10401209892823028No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG221210.10374945395641351No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC218680.10256286149445551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1513150.027.5040531
GGTATCA1031100.024.255271
GTATTAG205850.023.6364731
GTGGGTT253000.023.62974284
CATGCTA208250.023.3436954
GTACATG226900.022.4327981
TATTAGA212850.022.3621432
ATTAGAG214200.021.806413
ATGCTAA226400.021.4348475
TACATGC229400.021.3574662
ATCAACG1955150.021.280643
TAAGACT226300.021.221669
AACGCAG1967200.021.1265056
TCAACGC1977550.021.035314
CAACGCA1990200.020.9413175
TGCTAAG230500.020.8533366
ACATGCT241150.020.0536543
GAGGCAC221650.020.000337
ACGCAGA2073350.019.961877
TATCAAC2093450.019.9354042
Produced by FastQC (version 0.11.5)