FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6690091
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA1482912.216576725189538No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT1161641.7363590420518944No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG680091.0165631528778907No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG590820.8831270008135913No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG502610.7512752816067824No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC408390.6104401270475992No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT339410.5073324114724299No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC318200.47562880684283665No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA316260.47272899576403365No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG311700.46591294498086794No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC271760.4062127107090172No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC262260.39201260491075535No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT241010.3602492103620115No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT235150.3514899872064521No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT225870.3376187259635183No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT216670.32386704455888565No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA213380.31894932370875073No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA206170.3081721907818593No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT200380.29951759998481337No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG189340.2830155822992542No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA187480.2802353510587524No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG187070.279622504387459No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT186850.27929365983213084No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA185670.2775298572171888No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG183800.27473467849689936No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA180980.2705194891967837No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA174830.2613267891273826No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT174070.26019078066352164No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG171780.25676780779215114No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA162680.24316560118539493No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT158420.23679797479585854No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA146960.21966816295921834No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG145730.21782962294533809No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC143790.21492981186653515No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA143390.21433191267502938No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA143340.21425717527609114No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA141930.21214958062603334No Hit
GACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG139430.2084127106791223No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA139400.20836786823975936No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT134510.20105855062360137No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC133180.1990705358118447No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAAC129010.1928374367403971No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC128650.1922993274680419No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA127570.19068499965097635No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTAT126260.18872687979879496No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATC119730.17896617549746333No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT118640.17733690020061013No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC117440.17554320262609283No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC115720.17297223610261805No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT113250.16928020859506993No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT111970.1673669311822515No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG110170.16467638482047553No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA109150.16315174188213583No Hit
GGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCT104500.1562011637808813No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA103960.15539399987234853No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC103020.15398893677231No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC98670.14748678306468477No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACA97170.14524466109653814No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT96790.14467665686460768No Hit
CCCCTGAACTCTACACAACATATTTTGTCACCAAGACCCTACTTCTAACC96030.1435406484007467No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT95520.14277832693157685No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC95320.14247937733582397No Hit
GTATATAGGCTGAGCAAGAGGTGGTGAGGTTGATCGGGGTTTATCGATTA95230.1423448500177352No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG94970.14195621554325644No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT91740.13712817957184736No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGAAATTGAA91030.13606690850692466No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG87330.1305363409854963No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG87290.13047655106634573No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA85910.12841379885565085No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATC85540.127860742103508No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG85450.1277262147854192No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA84020.1255887251757861No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA83740.12517019574173205No Hit
CCCCTATACTGGGCCCTATTCAGTGGCAGCTTCTTGTTCCATAGGATTAA83190.12434808435341164No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT81480.12179206530972449No Hit
GTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCC81360.12161269555227276No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC80160.11981899797775546No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG79250.11845877731707984No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC78420.11721813649470537No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT77880.1164109725861726No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG77620.11602233811169385No Hit
CCTCTAGAGGGATATGAAGCACCGCCAGGTCCTTTGAGTTTTAAGCTGTG76580.11446780021377885No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC76270.1140044283403619No Hit
AGATTGAAGAGTTCAAATCTGACCAAGATGTTGATGTTGGATAAGAGAAT76030.11364568882545843No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGAT73220.10944544700513043No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG72020.10765174943061313No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT71630.107068797718895No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC70540.1054395224220418No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG70010.10464730599329666No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA69920.10451277867520785No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT69800.10433340891775614No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA69500.1038849845241268No Hit
GGGCAGCACAGTCATTTAAACTTGATCCAACCTCTTTGCATCTTACAAAG68810.10285360841877934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC108050.066.1628653
GGTATCA37350.066.0663451
ACTAAGT96850.064.7183158
GTATAAT114950.060.5476651
TCCGGTC250250.060.1750538
CCGGTCT249850.060.1606379
TACTAAG105650.059.982517
AGAACGG8350.058.5499842
AATACTA113550.057.92155
CTCCGGT264700.056.9075167
CGGATGC12050.056.6476679
TACCTTC112350.056.0770347
ACTCCGG266850.055.9305046
TACTCCG269800.055.2505725
ATACCTT107350.054.908076
TAATACT164850.054.7251934
CTAAGTT117750.053.7795339
TATACTG20200.053.4283685
CCTTCTG118000.052.766619
GGATTAC288650.051.8513761
Produced by FastQC (version 0.11.5)