FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278449
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences17216427
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA859520.49924412306920596No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT807200.4688545422345763No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC774160.44966356840475663No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA710940.4129428248962459No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT461000.26776752226231376No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA427960.24857654843249416No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT362810.2107347825422778No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC345610.20074432401101577No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC338930.19686430872096747No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA331030.19227566788393433No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT326420.18959799266131122No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA323220.18773930270200662No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG312850.18171598555263527No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT309690.17988052921782202No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG306810.17820770825444793No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT299280.17383397844395937No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC290650.16882132395995986No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG290000.16844377756197612No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG285050.16556861653117688No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT283710.1647902901107181No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA283460.16464507995764743No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT279770.16250177809832433No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT275890.16024811652266757No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA272750.15842427700009995No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTG270670.157216128526552No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT262690.15258102044053626No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA262470.15245323550583403No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC259770.15088496585267083No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA253460.14721986158916714No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG251480.14606979717684745No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA247190.14357799095015475No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC239700.1392274947641575No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG238500.1385304860294183No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC230580.13393022838013952No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT227780.132303874665748No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCT224920.13064267051461956No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG224490.13039290905133802No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT223860.1300269794655999No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC221710.12877817214919215No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA213840.12420695653052749No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC211280.12272000456308384No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT207820.12071029604458579No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC199730.11601129549121893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199480.11586608533814827No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG198500.11529686153811125No Hit
GGTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT195580.11360080695024583No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT186000.10803635388457779No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG185400.10768784951720818No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC185330.1076471906743484No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA184440.10713024252941683No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG181090.10518442647826985No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTT177350.10301208258833264No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT172680.10029955692897254No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC172570.10023566446162147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA284450.050.491171
GTATCAA622750.044.344861
ATCAACG878300.031.2934283
TCAACGC884150.031.0132394
CAACGCA901050.030.4265425
AACGCAG906100.030.1697656
TATAACG4500.028.9293142
ACGCAGA950850.028.6540857
CGCAGAG950750.028.502728
TATTAGA271600.027.645642
GTATTAG283250.027.5319671
ATTAGAG277850.027.2805543
GTGGGTT308150.027.17654684
CATGCTA278700.026.8943564
GTACATG284750.026.8412591
GCAGAGT998400.026.4114999
GAGGCAC279650.026.135927
TAAGACT293700.025.3600629
TATCAAC1094750.025.1754022
ATGCTAA299650.025.0698015
Produced by FastQC (version 0.11.5)