FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005278451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6208728
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA996221.6045476625808057No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT697801.123901707402869No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG493590.7949937571753827No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT307520.4953027415599459No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC285560.459933177939185No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA277360.44672596383671503No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG262550.4228724466589614No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG257970.41549573439197207No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC255560.4116141019545388No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC236110.3802872343578266No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC222780.3588174582619822No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG218730.35229438300405497No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT204200.3288918438688247No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT202150.32559004034320715No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA185790.2992400375729135No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA178670.28777231020589084No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA178140.28691867319682873No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA175990.28345580608459575No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA174500.281055958644025No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT174400.2808948950574095No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA174110.2804278106562246No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG170620.2748066914833441No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT168990.27218135502151164No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT167770.2702163792648027No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG166940.26887955149589415No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATC159460.2568319952170557No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA155520.2504860899044056No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA155470.2504055581110978No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT154190.24834394420241956No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTAT146160.23541053819719593No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG140070.22560176577231275No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC128620.20715998510483952No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC127720.20571041282530012No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA126950.20447022320836086No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAAC125590.20227975843039023No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA124450.2004436335429737No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC120300.19375949469843098No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA119960.19321187850393834No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG114650.18465940205465597No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT113540.18287159624322405No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA112940.18190521472353113No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA112820.18171193841959254No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG111890.18021404706406852No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC109620.17655790364789695No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT107900.1737876099581106No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG107510.17315946197031018No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC106770.17196759142935558No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT105970.17067908273643168No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA104160.16776383181869137No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC101210.1630124560135345No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG99740.16064482129028684No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC99190.15975897156390165No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT98920.15932409988003984No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT96250.15502370211740632No Hit
GACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG95370.15360634255519004No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG94050.15148030321186562No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC91700.14769530892640167No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATC90820.1462779493641854No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT90240.14534378056181557No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG90000.1449572279539384No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC89660.14440961175944572No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT89150.14358818746770674No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA88420.1424124232854137No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGAAATTGAA85870.13830530182671877No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT85540.13777379199088766No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT84570.13621147520071744No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGAT83330.1342142867266854No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG81390.13108965314634496No Hit
GTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCC77260.12443772701912534No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT76820.1237290472380172No Hit
GGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCG74760.12041113735373815No Hit
CGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA74280.11963803213798382No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA73490.11836562980372146No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT72300.11644897312299717No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG70060.11284114878281028No Hit
CAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGATAACA69840.11248680889225618No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG69670.11221300079500987No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG69090.11127883199264005No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA67490.10870181460679225No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA67030.107960922108361No Hit
GGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGT66710.10744551863119145No Hit
TCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT66370.10689790243669879No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC65280.10514230934258997No Hit
CCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGAT64380.10369273706305059No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)