FastQCFastQC Report
Thu 12 May 2022
EGAF00005278453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278453
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences33442645
Sequences flagged as poor quality0
Sequence length90
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT1902120.5687708014721922No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA1818840.543868464949468No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC1492250.446211715610413No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1415380.4232260935102472No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA913070.2730256533237727No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT874710.261555268729492No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA751860.2248207341255454No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT733070.21920215939857626No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC703850.21046481221805272No Hit
TTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACTCC682130.20397011061774573No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA664080.19857281025469128No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT659920.19732888950619787No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT632610.18916266940010276No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG629340.18818487592712838No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT614110.18363081030223538No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT612840.18325105565065203No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG609950.18238688955374194No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC609380.1822164484896455No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG589950.1764065013398312No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC581620.17391566964873742No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA575580.17210959240813636No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA565220.16901175131333063No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT562570.16821934987498746No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT553610.16554013595515546No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT548970.1641526858895282No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG548810.1641048427838169No Hit
TTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTCGTACAGGGAGGAATTTG529390.15829788582810958No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT528480.15802577816437666No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG527340.15768489603618374No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA523070.1564080831525138No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC495020.14802058868250403No Hit
CTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCA477320.14272794511319303No Hit
ATTGAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCT471460.1409756913665172No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG464580.1389184378209319No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC454600.13593422410219047No Hit
ATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAGGGA442360.13227422651527712No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT431250.12895212086244973No Hit
TATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTC429670.12847967019355078No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA424260.12686197518168793No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC405110.12113575346686843No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG396240.11848345129399902No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG390530.11677605045892753No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCG389560.11648600163055285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386910.11569360019220967No Hit
GGTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATT384830.11507163981796296No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC379850.1135825231526992No Hit
ACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACC378620.1132147292775437No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA377850.11298448433130813No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC365960.10942914353813821No Hit
GATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTT362760.10847228142391249No Hit
CTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCTGGATTACT349430.10448635267934099No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)