FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6551936
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA291930.44556296032195675No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT223300.34081529489909546No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA198370.3027654726786098No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT196920.3005523863480962No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATC184440.2815045812413308No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG171340.2615104909449665No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG161530.24653781721921583No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC158240.2415164006486022No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT152620.2329387832848184No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT141390.21579881122159922No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC130960.19987985230624966No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC130400.19902514310274091No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA119170.18188517103952176No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA116330.17755057436458474No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC112560.1717965499052494No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA111420.17005660616953522No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC109000.16636304139722977No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG107600.164226268388458No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG106280.16221159669447321No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGAAATTGAA106250.1621658087014281No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT106020.1618147674214156No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA103580.1580906773204134No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT101640.15512972043682965No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT96230.14687261902436166No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC90110.1375318684431594No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG88250.13469301287436264No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT87230.13313622111082893No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA85110.12990053626897455No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT84160.12845058315587943No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT84120.12838953249848595No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA79430.12123134291910055No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT78810.12028505772950164No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT77340.11804144607029127No Hit
GGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAA76930.11741567683200814No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG76050.1160725623693516No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC75950.1159199357258679No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTAT73140.111631127043976No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72490.11063905386133198No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACT71160.1086091195029988No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC70890.10819702756559282No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG69550.10615183054291127No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG69000.10531238400375095No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA66850.10203091116885146No Hit
GTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCTACTA66520.10152724324535525No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC66470.10145092992361342No Hit
TTACTCACCCGTCCGCCACTCGTCAGCAAAGAAGCAAGCTTCTTCCTGTT66210.1010541006505558No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA65880.1005504327270596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76950.056.190461
GCTACCG23000.055.5738267
CTACCGT24200.051.8647048
GAACGGA8350.048.6318973
AGAACGG16450.047.407362
TAATACT46500.046.641224
GTACTGG48550.045.147741
TACCTTC40150.045.0524987
AGCCGGT27200.044.7871486
ACGTGTC68750.043.761618
ACCACGT68450.043.7511635
TGGTTCA49600.043.539415
TAGCCGG28350.043.4586835
CCACGTG69650.043.0636256
TAGCTAC30000.042.914235
TTCACTA50650.042.8190278
TATCTAT52600.042.6352587
ATCTATT52350.042.574438
TTAGCCG29250.042.2798964
CCGGTCT83600.041.9493949
Produced by FastQC (version 0.11.5)