FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3811791
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG59560.1562520085702495No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT48480.12718430784898754No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA48470.127158073462055No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGG101800.045.5405771
GTATAAT36350.042.4694631
GAGTACG56500.041.3201681
TACGGGA55600.040.188112
ATAATAC45550.039.739553
CTAAGTT21450.039.219289
AGTACGG63100.037.4988022
TAGTTCG17050.037.210567
TAATACT45100.037.1050344
CTCCGGT17250.036.779137
TACGGGG57200.035.692132
TCCGGTC18100.035.5749558
GTTCGCT17150.035.350829
AGTTCGC17050.035.284838
GTATAGT37500.035.2319371
TACTCCG19650.034.67825
ACTAAGT25000.034.592368
TACTAAG26550.033.985497
ACGGGGC18450.033.8926773
CTATACT35950.033.7349624
Produced by FastQC (version 0.11.5)