FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005278510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6474273
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA917361.416931290972747No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT547740.8460254919741568No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG459430.7096240767110068No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG320660.4952834086545316No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTT315060.48663378884393665No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC292260.4514174796150857No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC286220.4420882468193726No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC283450.4378097741630605No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG251110.3878582197568746No Hit
GAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAA246650.3809694154077222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242170.37404971955924626No Hit
ACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCACC228150.35239477853343537No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT227730.3517460570476407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207470.3204529682328811No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA191900.29640393600949483No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGACTCTA184690.28526755050335384No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT178980.2764480274464793No Hit
GATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAA178370.27550583671711093No Hit
GCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGA172230.26602214642478No Hit
GGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTT169460.2617436737684679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168890.2608632660377466No Hit
GTCGTAAACCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTA166630.2573725266141851No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCA164610.2542524851825062No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT163060.2518583939849308No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG162800.2514568044937246No Hit
GGGTAAGATTTGCCGAGTTCCTTTTACTTTTTTTAACCTTTCCTTATGAG162710.25131779274676863No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG162070.25032926476841494No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA161790.24989678377788518No Hit
GAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGT161350.249217170792767No Hit
GTACTATATCTATTGCGCCAGGTTTCAATTTCTATCGCCTATACTTTATC161140.2488928100498697No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG151450.23392587862760805No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCA149510.23092940319322341No Hit
GAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAA149190.2304351392040465No Hit
CTCTTGTCCTTTCGTACAGGGAGGAATTTGAAGTAGATAGAAACCGACCT149120.23032701895641408No Hit
GTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTAT144860.22374712960049722No Hit
GGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTC141240.21815576822293406No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGATGGATCTCGTATGCCGT140650.21724446899288927No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA136170.21032477314441325No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT133860.20675680497254287No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT132760.20505777250974744No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTC129730.2003777103622291No Hit
GGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTATTGTTGATATGGA129180.19952819413083137No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAAT128220.1980454021633008No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTC125740.19421485624718018No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG118340.18278500149746543No Hit
GGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAATAGGGTTTAC115290.17807404785062353No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA115190.17791959035400579No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT113900.17592708864763656No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG112910.17439795943112069No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCA112690.1740581529385616No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAAC112260.17339398570310519No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC104700.161716998958802No Hit
GGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATT103880.16045044748653634No Hit
CTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAAC100740.15560048209273844No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACCAGTAT100410.15509077235389981No Hit
CTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGT96470.14900514698715978No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG95810.14798572750948255No Hit
GTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGGTAG95790.147954836010159No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATC95040.14679640478552572No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG93980.1451591553213774No Hit
CTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT91490.14131316365559501No Hit
CCTCCGAGCAGTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATA89870.13881095221038717No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC88930.13735905174218016No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG88860.13725093149454773No Hit
ACCTTAGCCAAACCATTTACCCAGATAAAGTATAGGCGATAGAAATTGAA88200.13623151201687048No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT86800.13406910706422173No Hit
GAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAAT85070.13139699237273436No Hit
CATTCATACAGGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCA83720.12931181616839452No Hit
AAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAAC83650.12920369592076208No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGA82290.12710307396676043No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGT81270.12552760750125921No Hit
GAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTT80250.123952141035758No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79680.1230717333050367No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAC79410.12265469806416875No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCAC79160.12226855432262433No Hit
GACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTG78970.12197508507905057No Hit
GTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCC78720.12158894133750615No Hit
GGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGG78560.12134180934291773No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT78300.12094021985171154No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT76980.11890138089635702No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA76680.11843800840650372No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGAT76450.11808275616428286No Hit
GGTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCG74410.11493182323328038No Hit
CGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA73590.11366527176101471No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73580.11364982601135293No Hit
GGGTAACTTGTTCCGTTGGTCAAGTTATTGGATCAATTGAGTATAGTAGT72810.11246050328739612No Hit
GCTCCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGG72670.11224426279213126No Hit
GGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGGTA71980.11117850606546867No Hit
GGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCT71630.11063790482730648No Hit
GTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTG71490.11042166433204162No Hit
GGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTT71250.11005096634015897No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA70560.10898520961349638No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC70420.10876896911823149No Hit
CTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAG67960.10496931470143442No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA66690.10300770449438879No Hit
GCTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTG66410.10257522350385903No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA65960.10188016476907909No Hit
GAGTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGG65520.10120055178396091No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)