FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005278521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005278521
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences14444740
Sequences flagged as poor quality0
Sequence length90
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT370080.2562039884414673No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG365500.25303328408818715No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA340650.23582978994429807No Hit
GTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTTTAATAGAAATTGGAC328240.22723842727525728No Hit
GTGTGGCAAAGGTGCCCTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTA307260.21271410908053728No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA278120.192540675706174No Hit
TCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACCTT276090.19113531984653237No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC273800.1895499676698923No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT252190.17458950455321454No Hit
CTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAACAAAGGAACC224160.1551845169937292No Hit
CTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG222770.1542222289913145No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC220590.1527130291026353No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT208060.14403859121036447No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA206480.1429447674378355No Hit
GCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTTAGGGAA173600.12018215627280242No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA168370.11656146112702617No Hit
GTGCTCACAGAAGCCAGGAACTTGTCCAGGGAGGCGTGCACCGCAGGGGT164430.11383382463097294No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC163260.11302384120447997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162400.11242846877133128No Hit
CTTGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAG159910.1107046578893078No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT157760.10921622680643611No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG152100.10529784544408553No Hit
GTATCAACGCAGAGTACGGGACATTTGCTTCTGACACAACTGTGTTCACT148220.10261174655964733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA274200.041.3473661
GTATCAA547100.040.3792721
TATTAGA149950.028.9758322
ATTAGAG153350.028.5217973
ATCAACG783100.028.3795853
GTATTAG162300.028.1279661
TCAACGC796250.027.941644
CAACGCA815650.027.352095
AACGCAG821650.027.2169086
GTACATG179000.027.2131021
CATGCTA180700.026.5756684
ACGCAGA855700.025.971787
CGCAGAG857500.025.9125048
TAAGACT186500.025.8071659
ACATGCT192100.025.0622773
GAGGCAC170300.024.988237
ATGCTAA193200.024.9195825
GTGGGTT201850.024.74330184
GCAGAGT851400.024.6614639
TGCTAAG195150.024.6078576
Produced by FastQC (version 0.11.5)