FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31875286
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA454600.010.45549494-95
GATCGGA497100.010.287909594-95
AGATCGG568600.010.113811594-95
TCGGAAG444250.09.91309394-95
TAGATCG177350.09.76389794-95
TATGCCG34400.08.69844348-49
CGTATGC34400.08.56037246-47
CGGAAGA437250.08.42032394-95
TCGTATG34550.08.38481644-45
CTCGTAT34350.08.2953844-45
AAGATCG206700.08.10168894-95
CAGATCG200350.07.69453294-95
ACACGTC149600.07.49440794-95
CACACGT195800.06.999870394-95
GAGATCG250950.06.938149594-95
GCACACG231900.06.72963594-95
CGTCTGA113600.05.93836594-95
CACGTCT166950.05.47773794-95
AGAGCAC415400.05.4437494-95
ACGTCTG141350.05.377507794-95
Produced by FastQC (version 0.11.5)