FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24063350
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA221300.010.49397194-95
ATCGGAA207950.010.11712694-95
AGATCGG258900.09.87793394-95
TAGATCG75600.09.42284194-95
GCGTCGT66950.08.37035594-95
CGCCGTA17500.08.27823646-47
AGCGTCG74700.08.20127894-95
TCGGAAG218000.07.951494794-95
AGAGCGT120800.07.64654294-95
CGTCGTG68200.07.625040594-95
GAGCGTC108800.07.485962494-95
AAGAGCG145350.07.22087294-95
CAGATCG121300.06.851562594-95
GCCGTAT22400.06.785439546-47
AAGATCG102550.06.576049394-95
GTCGTGT65500.06.5617594-95
CGTATCA24450.06.50809948-49
CGGAAGA230800.06.430231694-95
TCGTGTA56950.06.212621794-95
GAGATCG144200.05.467077794-95
Produced by FastQC (version 0.11.5)