FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99932634
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGAGCCCGCCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT1090650.1091385222569036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGCC532400.030.2918261
CGATAAC187400.029.52882438-39
GCGATAA188300.029.47661236-37
ATAACCG193900.028.6366440-41
TAAGACG204250.027.38051464-65
TAACCGG205550.027.12913740-41
AGCCCGC598400.026.9746344
AGCGATA208800.026.537136-37
CGGAGTA230200.024.01637344-45
CGACCAA244350.023.0477770-71
GAGCCCG740000.021.9221293
AACCGGA290350.019.32713742-43
ATCTCGA294500.019.09418718-19
GAGTCGC301450.018.78266128-29
AGACGAC301200.018.77417266-67
TTCGCGT91700.018.43806818-19
CGCCGGC628350.018.0432158
CCCGCCG722350.017.9176866
CCGCCGG632400.017.9126427
AGTCGCC317700.017.79204628-29
Produced by FastQC (version 0.11.5)